![]() Here, we will describe the five must-dos when evaluating a PCR master mix:Ī good PCR master mix should contain all of the components for your PCR reaction that are not specific to the sample itself. Whether you are designing small- or large- scale PCR assays for cloning, genotyping, or gene expression quantification, the master mix you choose is an essential component of your experimental design. Furthermore, depending on the goals of your experiment, there are various master mix reagents available to streamline your protocol and reduce pipetting steps and variability. Your experimental samples are precious and your work is important, therefore you should not have to spend unnecessary time and energy troubleshooting assay reagents or optimizing your master mix components. When it comes to PCR, the success of your assay is dependent upon the quality and reliability of the reagents you use. ![]() Universal ProbeLibrary Assay Design Center ![]()
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